ORGANIC FREE RADICALS IN BIOLOGICAL AND POLYMERIC SYSTEMS
For years the research group deals with the Study of Reaction Mechanisms involving Organic Free Radicals in different fields, like the Biological and Polymeric ones, where these species are considered as the main responsible of Autooxidation processes. The knowledge acquired is also exploited in the Study of Free Radical Polymerization with the aim to obtain new environmentally friendly materials, in a Circular Economy perspective.
The research activity carried out in the Bionanotechnology laboratory concerns the design and characterization of liquid crystalline nano-systems for a variety of hydrophobic, and hydrophilic small molecule drugs, peptide, proteins, DNAs and RNAs. These lyotropic systems are interesting soft materials thanks to their rich polimorfism as a function of concentration and temperature. They can be used as drug or gene delivery vectors, and in some cases for the controlled or targeted delivery to specific receptors. Liposomes, hexosomes and cubosomes represent carriers currently used in our research. Particularly the research activity is developed through:
The research activity for the study of the structure of matter is carried out not only in the Bionanotechnology Lab of the SIMAU Department but also in some large international facilities, particularly the European Synchrotron Radiation Facility (ESRF), (Grenoble – France), the Elettra Synchrotron (Trieste – Italy), ALBA synchrotron (Barcelona-Spain) e ISIS neutron source (Oxford- England)
Another activity carried out in the lab concerns the synthesis, characterization and optimization of molecularly imprinted polymers for the removal of Diclofenac (DCF) or other contaminants from waste water.
Liposomes for targeted gene and drug delivery
E. Crucianelli, P. Bruni,A. Frontini, L. Massaccesi, M. Pisani, A. Smorlesi and G. Mobbili, RSC Advances, 2014, 4, 58204
Innovative nanomaterials for applications in materials science and engineering, biology and nanomedicine
Astolfi, P.; Giorgini, E.; Gambini, V.; Rossi, B.; Vaccari, L.; Vita, F.; Francescangeli, O.; Marchini, C.; Pisani, M. Langmuir 2017, 33 (43), 12369–12378.
Astolfi, P.; Giorgini, E.; Adamo, F. C.; Vita, F.; Logrippo, S.; Francescangeli, O.; Pisani, M. J. Mol. Liq. 2019, 286, 110954.
APPLICATION OF INFRARED SPECTROSCOPY FOR THE STUDY AND CHARACTERIZATION OF ORGANIC, INORGANIC AND BIOLOGICAL COMPOUNDS
The research activity carried out at the Infrared Spectroscopy Laboratory is developed through the application of FTIR spectroscopy in the medium and near infrared for the study and characterization of organic, inorganic and biological compounds in different physical states. The coupling between IR spectroscopy and visible microscopy allows to analyze micron-sized samples and to obtain colorimetric maps in which each pixel corresponds to an infrared spectrum. The large amount of data acquired on a single sample is then subjected to statistical and multivariate analysis. The field of applicability is very wide: both organic and inorganic substances can be effectively analyzed.
FTIR Imaging of biological samples
This experimental activity is carried out in collaboration with the ELETTRA Research Center (Trieste).
FTIR of biocompatible materials
FTIR of materials in engineering
FTIR of synthetic polymers
Analysis and characterization of biodegradable polymers, obtained from natural molecules, to identify the presence of anomalies related to their working process and to characterize the products obtained by possible oxidative processes or by radical reactions, which can induce a variation of the crystalline lattice of the starting material.
FTIR of Microplastic
Analysis and characterization of microplastics obtained from the seabed, beaches and purification plants, to assess their degree of conservation.
THEORETICAL STUDIES (IN SILICO) OF COMPLEX MACROMOLECULAR SYSTEMS AND MOLECULES WITH ANTIOXIDANT ACTIVITY
The research activity is based on theoretical (in silico) studies of natural and synthetic polymeric systems, and of the behavior of molecules with antioxidant activity through an approach based on the DFT (Density Functional Theory) method and on Molecular Dynamics simulations (Molecular Dinamica ). The combined use of these techniques allows us to study radical species, small molecules and solid and liquid systems at an atomistic level, simulating their behavior over time, in order to provide useful tools for predicting the stability and the structured and electronic properties of complex systems. This detailed method provides to clarify the experimental macroscopic evidence, to stimulate and identify the best experimental conditions with regard to obtaining complex macromolecular systems, controlled the optimization of time and costs, including consumption and purchases, at the same time, a method valid for the identification of physico-chemical characteristics with a high level of reliability.